Total dilution factor for the second tube = dilution of first tube × dilution of the second tube.įor the first tube, dilution factor = 10 -1 (1 ml added to 9 ml)įor the second tube, dilution factor = 10 -1 (1ml added to 9 ml).After the first tube, each tube is the dilution of the previous dilution tube.In this case, the dilution factor for that test tube will be: The dilution factor of each tube in a set:įor a ten-fold dilution, 1 ml of sample is added to 9 ml of diluent.The dilution factor in a serial dilution can be determined either for an individual test tube or can be calculated as a total dilution factor in the entire series.Serial two-fold and ten-fold dilutions are commonly used to titer antibodies or prepare diluted analytes in the laboratory.In contrast, for a less contaminated sample, a low dilution factor might be sufficient. For e.g., if a water sample is taken from an extremely polluted environment, the dilution factor is increased. Depending on the estimated concentration of cells/organisms in a sample, the extent of dilution is determined.
#SERUM SERIAL NUMBER PROBLEM SERIES#
Then, a small measured volume of each dilution is used to make a series of pour or spread plates.Serial dilution involves the process of taking a sample and diluting it through a series of standard volumes of sterile diluent, which can either be distilled water or 0.9 % saline.
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